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In this chapter, you will be taken through the details of the three main parts of the Bacto-Aid project; spidersilk, bacteriocins and bio-degradable plastic (PHB).<br></p>
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Our project faces the growing problems of both plastic pollution and the evolution of antibiotic resistant bacterias. Bacto-Aid is a sustainable concept with bacteria aiding us. We focused on producing a bandage, which consists of recombinant spider silk integrated with antimicrobial peptides, making it preventive towards infections. The plastic of which we wish to attach the spider silk to is biodegradable and produced in the lab as well.<br></p>
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<p>In order to realize our project we set out to; 1) test the antimicrobial effects of different bacteriocins on different strains of bacteria, 2) assemble a silk expressing gene-constructs, and 3) optimize the PHB production. Additionally, we tried to create a hybrid silk gene-construct, in which bacteriocins are incorporated between the silk monomers.</p><br>
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<p>Scientific reproducibility has been a cornerstone of over project from the start, since we have been using other iGEM projects as basis for the development of our Bacto-Aid. We have likewise focused on reproducibility of our own project in order to assure that the good ideas lives on. Our results will first become interesting the moment reproducibility is assured. </p><br>
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<p>So let’s get down to the details.</p>
We will give you a thorough description of the project as a whole and its separate sub-elements. Through this, we will highlight our motivation that kept us working on Bacto-Aid, through sunnydays and rainy nights. Additionally you will get a more detailed insight into the experiments of the project, along with protocols of our laboratory work and at last but not least, our results. We will also try to prove our concept to you and give a better understanding of what scientific reproduction really is.
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Come on, click next. There is so much to discover!
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Revision as of 17:43, 19 October 2016

Project


Our project faces the growing problems of both plastic pollution and the evolution of antibiotic resistant bacterias. Bacto-Aid is a sustainable concept with bacteria aiding us. We focused on producing a bandage, which consists of recombinant spider silk integrated with antimicrobial peptides, making it preventive towards infections. The plastic of which we wish to attach the spider silk to is biodegradable and produced in the lab as well.

In order to realize our project we set out to; 1) test the antimicrobial effects of different bacteriocins on different strains of bacteria, 2) assemble a silk expressing gene-constructs, and 3) optimize the PHB production. Additionally, we tried to create a hybrid silk gene-construct, in which bacteriocins are incorporated between the silk monomers.


Scientific reproducibility has been a cornerstone of over project from the start, since we have been using other iGEM projects as basis for the development of our Bacto-Aid. We have likewise focused on reproducibility of our own project in order to assure that the good ideas lives on. Our results will first become interesting the moment reproducibility is assured.


Bacteriocins


Spider silk


poly-β-hydroxybutyrate PHB


So let’s get down to the details.