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<h4 align="center"> Although the researchers were able to add an inhibitor to their system to resist this enzyme there were still some issues with this approach. There was only a 50% chance of their edited strand being chosen as the template in cellular mismatch repair and the desired edit occurring. Also, the researchers could only perform a C to U edit, so the capabilities were limited. </h4> | <h4 align="center"> Although the researchers were able to add an inhibitor to their system to resist this enzyme there were still some issues with this approach. There was only a 50% chance of their edited strand being chosen as the template in cellular mismatch repair and the desired edit occurring. Also, the researchers could only perform a C to U edit, so the capabilities were limited. </h4> | ||
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<h4 align="center"> Based off of this, we started to think about editing the mRNA. But is targeting the RNA with CRISPR/Cas9 possible? </h4> | <h4 align="center"> Based off of this, we started to think about editing the mRNA. But is targeting the RNA with CRISPR/Cas9 possible? </h4> | ||
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− | <h4 align="center"> A paper published in 2014 showed that it is possible to target RNA with CRISPR through the use of a PAMmer. This is a short oligonucleotide that is separate from Cas9 but helps to guide the system there and activates nuclease | + | <h1 align="center"> Targeting RNA </h1> |
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+ | <h4 align="center"> A paper published in 2014 showed that it is possible to target RNA with CRISPR through the use of a PAMmer. This is a short oligonucleotide that is separate from Cas9 but helps to guide the system there and activates nuclease to performs the cleavage. Through the use of a PAMmer, dCas9 was also shown to be able to target mRNA. </h4> | ||
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<img align="center" src="https://static.igem.org/mediawiki/2016/6/63/PammerTrans.gif" alt = "pammer" /> | <img align="center" src="https://static.igem.org/mediawiki/2016/6/63/PammerTrans.gif" alt = "pammer" /> |
Revision as of 20:25, 19 October 2016