Difference between revisions of "Team:Austin UTexas/Protocols"
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<li> <span style="color:red;">16 g of Agar (if making solid media)</span> </li> | <li> <span style="color:red;">16 g of Agar (if making solid media)</span> </li> | ||
<li> Add distilled water to reach final volume </li> | <li> Add distilled water to reach final volume </li> | ||
| + | <li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | ||
| + | |||
| + | <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> | ||
Revision as of 00:47, 15 July 2016
HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Materials
- 5 g of Yeast Extract
- 5 g of Peptone
- 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
- 1.5 g of Citric Acid
- 16 g of Agar (if making solid media)
- Add distilled water to reach final volume
- 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS
