Difference between revisions of "Team:Austin UTexas/Protocols"

HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure

• 5 g of Yeast Extract
• 5 g of Peptone
• 2.7 g of Sodium Phosphate, dibasic anhydrous (Na₂HPO₄)
• 1.5 g of Citric Acid
• 16 g of Agar (if making solid media)
• Add distilled water to reach final volume
• Autoclave the solution
• 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
• DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS



YPD Media
Introduction
This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
Procedure

• 10 g of Yeast Extract
• 20 g of Peptone
• 16 g of Agar (if making solid media)
• Add distilled water to reach final volume
• Autoclave the solution
• 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
• DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS



PBS (Phosphate Buffered Saline) Solution
Introduction
This is the recipe to make 500 mL of PBS which is used in a variety of procedures.
Procedure 1. Add 400 mL to an empty bottle 2. Add and dissolve each of the following compounds in the water:

• 4.0 g of Sodium Chloride (NaCl)
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0.1 g of Potassium Chloride (KCl)

0.72 g of Sodium Phosphate, dibasic anhydrous (Na₂HPO₄)

0.12 g of Potassium Phosphate, monobasic (KH₂PO₄) </li)

3. Autoclave the solution