Difference between revisions of "Team:Technion Israel/Basic Part"

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                                         We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of  
 
                                         We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of  
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                                         concept in the LBD replacing strategy for our S.Tar platform. PctA is a chemoreceptor  
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                                         concept, in the <a data-toggle="popover" data-trigger="click" data-original-title="Info:" data-html="true" data-content="Ligand Binding Domain"> LBD<i class="entypo-check"></i></button></a> replacing strategy for our S.Tar platform. PctA is a chemoreceptor  
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                                         found in the bacterium P. Aeruginosa which mediates chemotaxis toward amino acids and  
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                                         found in the bacterium <i>P. Aeruginosa</i> which mediates chemotaxis toward amino acids and  
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                                         away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature  
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                                         away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature,
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                                         and tested for chemotaxis response to attractants but not to repellents. Our experiments  
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                                         and tested for chemotaxis response to attractants, but not to repellents. Our experiments  
 
                                         show that the hybrid receptor sensitive to the PctA repellent, organic components. This  
 
                                         show that the hybrid receptor sensitive to the PctA repellent, organic components. This  
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                                         property is significant and can be used in various applications including detection of  
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                                         property is influential, and can be used in various applications, including detection of  
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                                         polluted water.  To learn more please check our Proof of concept page and the PctA part  
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                                         polluted water.  To learn more, please check our <a href="https://2016.igem.org/Team:Technion_Israel/Proof" >Proof of concept</a> page and the PctA part  
 
                                         ,<a>K1992001</a>, in the registry.
 
                                         ,<a>K1992001</a>, in the registry.
 
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Revision as of 21:13, 19 October 2016

S.tar, by iGEM Technion 2016

S.tar, by iGEM Technion 2016

Parts' Index


Best new basic part

We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of concept, in the LBD replacing strategy for our S.Tar platform. PctA is a chemoreceptor found in the bacterium P. Aeruginosa which mediates chemotaxis toward amino acids and away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature, and tested for chemotaxis response to attractants, but not to repellents. Our experiments show that the hybrid receptor sensitive to the PctA repellent, organic components. This property is influential, and can be used in various applications, including detection of polluted water. To learn more, please check our Proof of concept page and the PctA part ,K1992001, in the registry.


This is our basic parts:

Name Type Description Designer Length
Favorite U W BBa_K1992001 Coding PctA-Tar hybrid receptor Shilo Ohayon, Asif Gil 1920
Favorite W BBa_K1992002 Coding Histamin-Tar receptor Inbal Adir 1662
Favorite W BBa_K1992003 Tag Tar chemoreceptor tagged with GFP Shilo Ohayon 2400
W BBa_K1992000 RBS Tar native RBS Shilo Ohayon 24



S.tar, by iGEM Technion 2016