Line 198: | Line 198: | ||
<p class="text-justify"> | <p class="text-justify"> | ||
We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of | We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of | ||
− | concept in the LBD replacing strategy for our S.Tar platform. PctA is a chemoreceptor | + | concept, in the <a data-toggle="popover" data-trigger="click" data-original-title="Info:" data-html="true" data-content="Ligand Binding Domain"> LBD<i class="entypo-check"></i></button></a> replacing strategy for our S.Tar platform. PctA is a chemoreceptor |
− | found in the bacterium P. Aeruginosa which mediates chemotaxis toward amino acids and | + | found in the bacterium <i>P. Aeruginosa</i> which mediates chemotaxis toward amino acids and |
− | away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature | + | away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature, |
− | and tested for chemotaxis response to attractants but not to repellents. Our experiments | + | and tested for chemotaxis response to attractants, but not to repellents. Our experiments |
show that the hybrid receptor sensitive to the PctA repellent, organic components. This | show that the hybrid receptor sensitive to the PctA repellent, organic components. This | ||
− | property is | + | property is influential, and can be used in various applications, including detection of |
− | polluted water. To learn more please check our Proof of concept page and the PctA part | + | polluted water. To learn more, please check our <a href="https://2016.igem.org/Team:Technion_Israel/Proof" >Proof of concept</a> page and the PctA part |
,<a>K1992001</a>, in the registry. | ,<a>K1992001</a>, in the registry. | ||
<br> | <br> |
Revision as of 21:13, 19 October 2016
![](https://static.igem.org/mediawiki/2016/f/f6/T--Technion_Israel--biobrickscov.jpg)
Best new basic part
We chose PctA-Tar hybrid as our best new basic part. This part serves as the proof of
concept, in the LBD replacing strategy for our S.Tar platform. PctA is a chemoreceptor
found in the bacterium P. Aeruginosa which mediates chemotaxis toward amino acids and
away from organic compounds. The PctA-Tar hybrid was conducted previously in the literature,
and tested for chemotaxis response to attractants, but not to repellents. Our experiments
show that the hybrid receptor sensitive to the PctA repellent, organic components. This
property is influential, and can be used in various applications, including detection of
polluted water. To learn more, please check our Proof of concept page and the PctA part
,K1992001, in the registry.
This is our basic parts:
Name | Type | Description | Designer | Length | |||
---|---|---|---|---|---|---|---|
Favorite | U | W | BBa_K1992001 | Coding | PctA-Tar hybrid receptor | Shilo Ohayon, Asif Gil | 1920 |
Favorite | W | BBa_K1992002 | Coding | Histamin-Tar receptor | Inbal Adir | 1662 | |
Favorite | W | BBa_K1992003 | Tag | Tar chemoreceptor tagged with GFP | Shilo Ohayon | 2400 | |
W | BBa_K1992000 | RBS | Tar native RBS | Shilo Ohayon | 24 |
![](https://static.igem.org/mediawiki/2016/5/5a/T--Technion_Israel--up_arrow.png)