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<h3>Experiment </h3> | <h3>Experiment </h3> | ||
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<br> | <br> | ||
<h4> For in vivo using liquid cultures</h4> | <h4> For in vivo using liquid cultures</h4> | ||
− | <h5> | + | <h5>Cultures that contained strains with plasmids with a 500bp spacer or 1000bp spacer, were diluted to normalize their OD absorbance. RFP expression (in AFU), GFP expression (in AFU), and OD was measured in a plate reader (<a href="http://www.perkinelmer.com/product/victor-x5-for-fl-lum-uv-trf-fp-2030-0050">VICTOR-X3</a>). For strains using a 1000bp spacer we the effects of using inducer (ATc and IPTG) were tested as well. (See our <a href="https://2016.igem.org/Team:Alverno_CA/Protocols">Plate Reading (for Fluorescence, Absorbance, Induction, etc.) Protocol</a> for more information) </h5> |
<center><img src="https://static.igem.org/mediawiki/2016/1/1e/Cultures_Experiment_page.jpeg" alt="width:200px;"></center> | <center><img src="https://static.igem.org/mediawiki/2016/1/1e/Cultures_Experiment_page.jpeg" alt="width:200px;"></center> |
Revision as of 23:01, 19 October 2016