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<p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario. | <p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario. | ||
− | < | + | <figure><a="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" data-lightbox="img" data-title="Figure 1: We placed a microfluidic chip containing E. coli transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in E. coli at this temperature."><img src="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" width=100% /></a><figcaption>Figure 1: We placed a microfluidic chip containing E. coli transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in E. coli at this temperature</figcaption></figure></p> |
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Revision as of 23:50, 19 October 2016
Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario.