<p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario.
<p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario.
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<figure><a="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" data-lightbox="img" data-title="Figure 1: We placed a microfluidic chip containing <em>E. coli</em> transformed with our <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895000</a> construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42°C before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in <em>E. coli</em> at this temperature."><img src="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" width=100% /></a><figcaption>Figure 1: We placed a microfluidic chip containing <em>E. coli</em> transformed with our <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895000</a> construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42°C before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in <em>E. coli</em> at this temperature</figcaption></figure></p>
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<figure><a="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" data-lightbox="img" ><img src="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" width=100% /></a><figcaption>Figure 1: We placed a microfluidic chip containing <em>E. coli</em> transformed with our <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895000</a> construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shaking incubator at 42°C before being injected into the chip. This was due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that sfGFP is expressed highly in <em>E. coli</em> at this temperature.</figcaption> </figure></p>
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<figure><a="https://static.igem.org/mediawiki/2016/3/3b/T--Newcastle--igemdemonstrate.png" data-lightbox="img" data-title="Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli</em> transformed with <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895004</a> and another microfluidic chip containing 1M NaCl solution onto our breadboard, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery' was as we expected based on our previous results (LINK HERE). This confirms that two independent components can interact with one another within the breadboard system."><img src="https://static.igem.org/mediawiki/2016/3/3b/T--Newcastle--igemdemonstrate.png" width=100% /></a><figcaption>Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli </em> transformed with <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895004</a> and another microfluidic chip containing 1M NaCl solution, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery') was as we expected based on our previous results <a href="https://2016.igem.org/Team:Newcastle/Proof/MFC">results</a>.</figcaption></figure></p>
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<figure><a="https://static.igem.org/mediawiki/2016/3/3b/T--Newcastle--igemdemonstrate.png" data-lightbox="img" data-title="Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli</em> transformed with <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895004</a> <img src="https://static.igem.org/mediawiki/2016/3/3b/T--Newcastle--igemdemonstrate.png" width=100% /></a><figcaption>Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli </em> transformed with <a href="http://parts.igem.org/Part:BBa_K1895000">BBa_K1895004</a> and another microfluidic chip containing 1M NaCl solution, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery') was as we expected based on our previous results <a href="https://2016.igem.org/Team:Newcastle/Proof/MFC">results</a>.</figcaption></figure></p>
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Revision as of 00:39, 20 October 2016
Demonstrate
Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario.
Figure 1: We placed a microfluidic chip containing E. coli transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shaking incubator at 42°C before being injected into the chip. This was due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that sfGFP is expressed highly in E. coli at this temperature.
BBa_K1895004 Figure 2: We placed the miniature microbial fuel cell construct containing E. coli transformed with BBa_K1895004 and another microfluidic chip containing 1M NaCl solution, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery') was as we expected based on our previous results results.
