Difference between revisions of "Team:Marburg/Results"

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<p>Here you can describe the results of your project and your future plans. </p>
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        <meta name="description" content="iGEM Marburg 2016 Wiki">
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        <meta name="author" content="Martin Lellep, iGEM Team Marburg 2016" >  
  
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<h5>What should this page contain?</h5>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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        <link rel="icon" href="../../img/favicons/favicon.ico">
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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        <!-- Title -->
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        <title>Projects :: Syndustry - iGEM Marburg 2016</title>
  
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        <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:Marburg/CSS_Bootstrap?action=raw&ctype=text/css" />
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<h5> Project Achievements </h5>
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<div class="container">
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    <div class="master-heading">
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        <h1>
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            SynDustry <small>Fuse. Produce. Use.</small>
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        </h1>
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    </div>
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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</ul>
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<div class="container">
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    <div class="heading">
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        <h2>Results</h2>
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    </div>
 
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            <h3 class="text-center">Approaches towards artificial endosymbiosis</h3>
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            <p>
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                We had several subprojects towards achieving our big goal of establishing artificial endosymbiosis and providing the iGEM community with a list of fully charakterized kill switches.
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            </p>
  
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<ul>
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        </div>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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    </div>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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</ul>
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            <h2 class="featurette-heading">
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                Dependency.
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                <span class="text-muted">
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                    Keeps the system together.
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                </span>
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            </h2>
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            <p class="featurette-text">
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                In order to guarantee the fitness of  the host organism as well as the invading cell,
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                we established different exchange-based dependencies - a malonate based dependency with
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                the invading <i>E. coli</i> cell as the malonate source and a protein based dependency
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                complementing essential gene knockout.
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            </p>
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            <p class="text-center">
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                <a class="featurette-button btn btn-default" href="https://2016.igem.org/Team:Marburg/Dependency" role="button">More details &raquo;</a>
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            </p>
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        </div>
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        <div class="col-md-5">
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            <img class="featurette-image img-fluid m-x-auto" src="https://static.igem.org/mediawiki/2016/a/a9/T--Marburg--img_Dependency_small.png" alt="Generic placeholder image">
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            <img class="featurette-image img-fluid m-x-auto" src="https://static.igem.org/mediawiki/2016/2/29/T--Marburg--img_PEG_small.png" alt="Generic placeholder image">
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        </div>
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        <div class="col-md-7">
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            <h2 class="featurette-heading">
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                PEG Method.
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                <span class="text-muted">
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                    Brings the system together.
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                </span>
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            </h2>
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            <p class="featurette-text">
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                Since our whole project relies on the utilization of the principles of endosymbiosis for production purposes, we had to decide for a method to bring the organisms together. This was accomplished by modification and optimization of a protocol using <i>polyethylene glycol</i> (PEG).
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            </p>
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            <p class="text-center">
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                <a class="featurette-button btn btn-default" href="https://2016.igem.org/Team:Marburg/PEG_Method" role="button">More details &raquo;</a>
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            </p>
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        </div>
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    </div>
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            <h2 class="featurette-heading">
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                Production Line.
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                <span class="text-muted">
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                    Novel system for modular synthesis.
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                </span>
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            </h2>
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            <p class="featurette-text">
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                Design of a production line that starts with limonene production in E. coli and export in S. cerevisiae.                                         
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                Limonene gets hydroxylated to the cancer drug perillyl alcohol and exported to the media.
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            </p>
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            <p class="text-center">
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                <a class="featurette-button btn btn-default" href="https://2016.igem.org/Team:Marburg/Production" role="button">More details &raquo;</a>
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            </p>
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            <img class="featurette-image img-fluid m-x-auto" src="https://static.igem.org/mediawiki/2016/c/c0/T--Marburg--img_Production_small.png" alt="Generic placeholder image">
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        <div class="col-md-7">
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            <h2 class="featurette-heading">
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                Modeling.
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                <span class="text-muted">
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                    Evolutionary stability analysis of killswitches.
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                </span>
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            </h2>
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            <p class="featurette-text">
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                Quantitative work using mathematical modeling and numerics to study genetical killswitches. Treated as
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                genetic regulatory networks, the stability of network topologies against evolution - selection and mutation -
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                is studied.
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            </p>
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            <p class="text-center">
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                <a class="featurette-button btn btn-default" href="https://2016.igem.org/Team:Marburg/Modeling" role="button">More details &raquo;</a>
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            </p>
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        </div>
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    </div>
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    <hr class="featurette-divider">
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    <h3 class="text-center">Literature</h3>
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    <ol class="literature_list">
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        <li id="ref_1"><a href="#ref_1" class="ref">[1]</a> Ajikumar, Parayil Kumaran, et al. "Terpenoids: opportunities for biosynthesis of natural product drugs using
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        engineered microorganisms." Molecular pharmaceutics 5.2 (2008): 167-190.</li>
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        <li id="ref_2"><a href="#ref_2" class="ref">[2]</a> Van Dien, Stephen. "From the first drop to the first truckload: commercialization of microbial processes for
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        renewable chemicals." Current opinion in biotechnology 24.6 (2013): 1061-1068.</li>
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    </ol>
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Revision as of 02:36, 20 October 2016

Projects :: Syndustry - iGEM Marburg 2016

SynDustry Fuse. Produce. Use.

Results

Approaches towards artificial endosymbiosis

We had several subprojects towards achieving our big goal of establishing artificial endosymbiosis and providing the iGEM community with a list of fully charakterized kill switches.

Dependency. Keeps the system together.

In order to guarantee the fitness of the host organism as well as the invading cell, we established different exchange-based dependencies - a malonate based dependency with the invading E. coli cell as the malonate source and a protein based dependency complementing essential gene knockout.

More details »

Generic placeholder image
Generic placeholder image

PEG Method. Brings the system together.

Since our whole project relies on the utilization of the principles of endosymbiosis for production purposes, we had to decide for a method to bring the organisms together. This was accomplished by modification and optimization of a protocol using polyethylene glycol (PEG).

More details »

Production Line. Novel system for modular synthesis.

Design of a production line that starts with limonene production in E. coli and export in S. cerevisiae. Limonene gets hydroxylated to the cancer drug perillyl alcohol and exported to the media.

More details »

Generic placeholder image
Generic placeholder image

Modeling. Evolutionary stability analysis of killswitches.

Quantitative work using mathematical modeling and numerics to study genetical killswitches. Treated as genetic regulatory networks, the stability of network topologies against evolution - selection and mutation - is studied.

More details »

Literature

  1. [1] Ajikumar, Parayil Kumaran, et al. "Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms." Molecular pharmaceutics 5.2 (2008): 167-190.
  2. [2] Van Dien, Stephen. "From the first drop to the first truckload: commercialization of microbial processes for renewable chemicals." Current opinion in biotechnology 24.6 (2013): 1061-1068.