Difference between revisions of "Team:Pasteur Paris/Scenarios"
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The development of our project’s concept and the design of our device (i.e. patch and trap) was an <B>iteractive process</B> that involved a multidimensional collaboration of people and ideas. We successfully achieved this through:</br> | The development of our project’s concept and the design of our device (i.e. patch and trap) was an <B>iteractive process</B> that involved a multidimensional collaboration of people and ideas. We successfully achieved this through:</br> | ||
| − | + | 1) numerous team brainstorming activities; </br> | |
| − | + | 2) oral and poster presentations to various audiences (i.e. scientists, young researchers, professionals, and students);</br> | |
| − | + | 3) organizing meetings with scientific experts (i.e. ecologists, entomologists); </br> | |
| − | + | 4) consulting local agencies (French Interdepartmental Mosquito Control Board (EID) and in the United States the New Orleans Mosquito Control Board) and a drone company to discuss our project and if applicable, potential collaboration.</br></br> | |
For more information go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Methodology">Methodology</a> setion.</br></br> | For more information go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Methodology">Methodology</a> setion.</br></br> | ||
| − | Additionally, we incorporated surveys assessing the general public’s understanding and acceptance of synthetic biology. These steps were essential to ensuring we gathered knowledge, gained feedback, and considered as many options as possible; making necessary adjustments based on our findings. Our tool will be user-friendly, safe, and applicable.</br> </br></br> | + | Additionally, we incorporated surveys assessing the general public’s understanding and acceptance of synthetic biology. These steps were essential to ensuring we gathered knowledge, gained feedback, and considered as many options as possible; making necessary adjustments based on our findings. Our tool will be <B>user-friendly</B>, <B>safe</B>, and <B>applicable</B>.</br> </br></br> |
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| − | The Mos(kit)o device is easy to use and it does not require scientific expertise so it is possible to train people locally. From the set up of the trap to the analysis of the patch, the steps are simple and designed to reduce user error. For example, the quick read function has a color change component to ensure quick and accurate interpretation of results; changing from no color to color for positive results.</br> </br> | + | The Mos(kit)o device is easy to use and it does not require scientific expertise so it is possible to <B>train people locally</B>. From the set up of the trap to the analysis of the patch, the steps are simple and designed to reduce user error. For example, the quick read function has a <B>color change</B> component to ensure quick and accurate interpretation of results; changing from no color to color for positive results.</br> </br> |
For more information go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Moskit_devices">Devices</a> section.</br></br></br> | For more information go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Moskit_devices">Devices</a> section.</br></br></br> | ||
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<B>In the Laboratory</B> | <B>In the Laboratory</B> | ||
| − | The students working on this project received laboratory safety training and were supervised during all experiments. All manipulations involving GMOs only took place in a contained biosafety level II laboratory and the usage of GMOs was only required for the production of the biosilica protein (made using E. coli bacteria). The protein was made in the cell and when the cell was lysed, the protein was released and the GMO destroyed. Therefore, there was no release of the GMO during this process. </br></br> | + | The students working on this project received laboratory <B>safety training</B> and were supervised during all experiments. All manipulations involving <B>GMOs</B> only took place in a <B>contained biosafety level II</B> laboratory and the usage of GMOs was only required for the production of the biosilica protein (made using <i>E. coli</i> bacteria). The protein was made in the cell and when the cell was lysed, the protein was released and the GMO <B>destroyed</B>. Therefore, there was no release of the GMO during this process. </br></br> |
| − | In the Field. All personnel using the Mos(kit)o will be trained to ensure proper usage of the device. The kits are designed to be safe and user friendly, requiring no scientific background. The operators will not have any contact with the infectious materials, reagents, or the newly produced protein. </br></br></br> | + | In the Field. All personnel using the Mos(kit)o will be trained to ensure proper usage of the device. The kits are <B>designed</B> to be safe and user friendly, requiring no scientific background. The operators will not have any contact with the infectious materials, reagents, or the newly produced protein. </br></br></br> |
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| − | Upon successful completion of our lab work and following informed discussions with stakeholders, we illustrated two application scenarios where the Mos(kit)o would be utilized: in developed countries and in developing countries. The diagram below explains the life cycle of our device, beginning with the production of the biosilica protein and concluding with real life applications and potential outcomes.</br></br> | + | Upon successful completion of our lab work and following informed discussions with stakeholders, we illustrated two <B>application scenarios</B> where the Mos(kit)o would be utilized: in <B>developed</B> countries and in <B>developing</B> countries. The diagram below explains the life cycle of our device, beginning with the production of the biosilica protein and concluding with <B>real life applications</B> and potential outcomes.</br></br> |
| − | <B>Context 1</B> describes developed countries where agencies or municipalities like the Centers for Disease Control and Prevention (CDC) in the US or the French Interdepartmental Mosquito Control Board (EID), respond to emergencies. A warning is received from a specific location and these agencies are responsible for distributing the Mos(kit)o and ensuring that appropriate personnel are deployed to endemic areas. </br></br> | + | <B>Context 1</B> describes developed countries where agencies or municipalities like the Centers for Disease Control and Prevention (<a href="https://www.cdc.gov"><B>CDC</B></a>) in the US or the French Interdepartmental Mosquito Control Board (<a href="http://www.eid-med.org"><B>EID</B></a>), respond to emergencies. A warning is received from a specific location and these agencies are responsible for <B>distributing</B> the Mos(kit)o and ensuring that appropriate personnel are deployed to endemic areas. </br></br> |
| − | Currently, the CDC has an Epidemic Intelligence Service (EIS) that responds during public health outbreaks. They are responsible for identifying the causal agent(s) and they are tasked with assisting endemic areas with efforts to prevent and/or control the transmission of the disease(s). 2 A similar institutional set-up can be found in France. Following our conversation with a division director for mosquito preparedness at EID (acronym), we developed a practical example illustrating the utility of the Mos(kit)o device. Presently, administrators manually test endemic areas, which is labor and time intensive. The design of our device eliminates these conditions by using stationary traps and a patch capable of simultaneously detecting multiple viruses. Additionally, the division director of EID expressed interested in our device and suggested the need to have these traps within certain distances from hospitals where infected people are treated or airports because it is a transit place for global entry/exit from endemic areas. Our device would be used as an important proactive measure that could protect non-infected people if they are in close proximity to these particular hot spots (e.g. hospitals, airports) where infected mosquitoes are circulating. In scenario 1, these organizations have the resources to respond to the hazards, administer the kits, and analyze the results; making them ideal for Mos(kit)o.</br></br> | + | Currently, the CDC has an Epidemic Intelligence Service (<a href="https://www.cdc.gov/eis"><B>EIS</B></a>) that responds during public health outbreaks. They are responsible for identifying the causal agent(s) and they are tasked with assisting endemic areas with efforts to prevent and/or control the transmission of the disease(s). 2 A similar institutional set-up can be found in France. Following our conversation with a division director for mosquito preparedness at EID (acronym), we developed a practical example illustrating the utility of the Mos(kit)o device. Presently, administrators manually test endemic areas, which is labor and time intensive. The design of our device eliminates these conditions by using stationary traps and a patch capable of simultaneously detecting multiple viruses. Additionally, the division director of EID expressed interested in our device and suggested the need to have these traps within certain distances from hospitals where infected people are treated or airports because it is a transit place for global entry/exit from endemic areas. Our device would be used as an important proactive measure that could protect non-infected people if they are in close proximity to these particular hot spots (e.g. hospitals, airports) where infected mosquitoes are circulating. In scenario 1, these organizations have the resources to respond to the hazards, administer the kits, and analyze the results; making them ideal for Mos(kit)o.</br></br> |
<B>In Context 2</B> , which describes developing countries where access is not as easy and there are no local agencies as mentioned in scenario 1, the Ministry of Health, or equivalent instance, would assume responsibility for administering the kits. Personnel at the local Ministry of Health will be trained to properly set up the trap and on how to use the diagnostic device. We are aware that resources are limited in these settings; therefore, we designed the device to require minimum personnel for operation and to be low cost, user friendly, and safe. We were unable to discuss this specific scenario with a health ministry official to get their feedback, however, based on a conversation with tropical entomologists and the New Orleans Mosquito Control Board, which is solicited by the Brazilian government, we envisioned a situation in which a country receives a report of an outbreak of a vector-borne disease, like zika virus. In that case, the Ministry of Health would respond by requesting a Mos(kit)o device, either from the Mos(kit)o company or possibly a local NGO or CDC, with a stationary trap (for easily accessible areas) or a drone (capable of reaching remote areas). Upon arrival of the kit, trained local authorities will implement the trap in targeted locations and perform the required tests. An additional authorized person reads the results and the data will be entered into mapping software, capable of producing real-time updates. </br></br> | <B>In Context 2</B> , which describes developing countries where access is not as easy and there are no local agencies as mentioned in scenario 1, the Ministry of Health, or equivalent instance, would assume responsibility for administering the kits. Personnel at the local Ministry of Health will be trained to properly set up the trap and on how to use the diagnostic device. We are aware that resources are limited in these settings; therefore, we designed the device to require minimum personnel for operation and to be low cost, user friendly, and safe. We were unable to discuss this specific scenario with a health ministry official to get their feedback, however, based on a conversation with tropical entomologists and the New Orleans Mosquito Control Board, which is solicited by the Brazilian government, we envisioned a situation in which a country receives a report of an outbreak of a vector-borne disease, like zika virus. In that case, the Ministry of Health would respond by requesting a Mos(kit)o device, either from the Mos(kit)o company or possibly a local NGO or CDC, with a stationary trap (for easily accessible areas) or a drone (capable of reaching remote areas). Upon arrival of the kit, trained local authorities will implement the trap in targeted locations and perform the required tests. An additional authorized person reads the results and the data will be entered into mapping software, capable of producing real-time updates. </br></br> | ||
