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A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq [[Team:Paris_Saclay/Experiments#taqPCR|usual]] protocol. We used puc19 universal [[Team:Paris_Saclay/Experiments#primers|primers]] 1151_pheoR and 1152_pheoF.
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Megamix were prepared for 34 tubes with 70μL of 10xTaq buffer + 70 μL of dNTP 10mM + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.)<br/>
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19 μL of megamix was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clones for 2.2).
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1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007.
