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Line 11: ===Biobrick characterization===
===Biobrick characterization===
− ==== ====
+ ==== DNA extraction of K1372001 clone 1 and clone 2 ====
− ''By ''
+ ''By Mathilde ''
+ Plasmids DNA were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|usual]] protocol except that isopropanol was used instead of 100% ethanol and that pellets were dried on the lab bench top instead of in the speedvac. The extraction were kept at -20°C.
===Visualization===
===Visualization===
Revision as of 15:01, 18 July 2016
Wednesday 6th July Lab work Preparation of LB solid and liquid stock By Léa, Naiane, Laetitia
- 2L of LB liquid : 20g/L powder LB + 2L water μQ
Biobrick characterization DNA extraction of K1372001 clone 1 and clone 2 By Mathilde
Plasmids DNA were extracted following the usual protocol except that isopropanol was used instead of 100% ethanol and that pellets were dried on the lab bench top instead of in the speedvac. The extraction were kept at -20°C.
Visualization gBlocks PCR screening By Caroline
A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF.
1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007.
pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks By Léa, Caroline and Laetitia
The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.
