Monday 18^th July

Lab work

Preparation of LB solid and liquid stock

By Caroline and Terrence

- 1L of LB liquid : 20g/L powder LB + 1L water μQ
- 500mL of LB solid : 500mL of LB liquid + 7.5g/L of Agar
The all was put in the autoclave for sterilization.

Getting DNA closer

Electrophoresis of PCR products DS_SPcasN, DS_TDcasN, pZA21 and pZA31

By Caroline

PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel.

Biobrick characterization

LB culture of electro-transformed BL21 cells (From the 13/07/2016)

By Mathilde

Each transformation : pclTAA + K1372001, pcl_TAG + K1372001 and pcl_Tq + K1372001 was added to 3mL of LB and one or two antibiotics.

Three conditions were tested for each trasnformation :

• 15µL of Chloramphenicol (30µg/mL)
• 5 µL of Streptomycin (50µg/mL)
• 15µL of Chloramphenicol (30µg/mL) + 5 µL of Streptomycin (50µg/mL)

Cultures were put in incubation at 37°c, 180 rpm.

Visualization

Electrophoresis of PCR products pPS16_004

By Caroline

PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel