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<h3>Testing spacers <i>in vivo</i></h3> | <h3>Testing spacers <i>in vivo</i></h3> | ||
− | <h5>Cultures | + | <h5>Cultures of strains containing plasmids with a 500bp spacer or 1000bp spacer were diluted to the same OD. RFP expression (in AFU), GFP expression (in AFU), and OD were measured using a (<a href="http://www.perkinelmer.com/product/victor-x5-for-fl-lum-uv-trf-fp-2030-0050">VICTOR-X3</a>) plate reader. Inducers - specifically ATc and IPTG - were added to the strains containing plasmids with the 1000bp spacer. (See <a href="https://2016.igem.org/Team:Alverno_CA/Protocols">"Plate Reading"</a> for more information) </h5> |
<h3> Testing dCas9 clamps <i>in vitro</i> </h3> | <h3> Testing dCas9 clamps <i>in vitro</i> </h3> |
Revision as of 03:19, 20 October 2016