Difference between revisions of "Team:Paris Saclay/Notebook/July/19"
(Created page with "{{Team:Paris_Saclay/notebook_header}} =Tuesday 19<sup>th</sup> July= ==Lab work== ===Biobrick characterization=== ===Visualization=== ==== Ligation of gBlock 1.2, 2.2, 4.1...") |
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==== Ligation of gBlock 1.2, 2.2, 4.1 within pUC19==== | ==== Ligation of gBlock 1.2, 2.2, 4.1 within pUC19==== | ||
''By Naiane & Charlène'' | ''By Naiane & Charlène'' | ||
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gBlock 1.2, 2.2, 4.1 were [[Team:Paris_Saclay/Experiments#Ligation|inserted]] in pUC19. | gBlock 1.2, 2.2, 4.1 were [[Team:Paris_Saclay/Experiments#Ligation|inserted]] in pUC19. | ||
Two controls were made : | Two controls were made : | ||
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====Transformation of DH5α with 1.2, 2.2, 4.1==== | ====Transformation of DH5α with 1.2, 2.2, 4.1==== | ||
''By Naiane & Charlène'' | ''By Naiane & Charlène'' | ||
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HeatShock competent cells were [[Team:Paris_Saclay/Experiments#heat-shocktransformation|transformed]] with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). | HeatShock competent cells were [[Team:Paris_Saclay/Experiments#heat-shocktransformation|transformed]] with 1.2, 2.2 and 4.1. Another control was made (cells without plasmid). | ||
Cells were plated on LB + Ampicillin + IPTG + Xgal : | Cells were plated on LB + Ampicillin + IPTG + Xgal : | ||
Revision as of 12:41, 19 July 2016
