Difference between revisions of "Team:Paris Saclay/Notebook/July/21"

(Biobrick characterization)
(Tuesday 19th July)
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=Tuesday 19<sup>th</sup> July=
 
=Tuesday 19<sup>th</sup> July=
 
==Lab work==
 
==Lab work==

Revision as of 12:31, 21 July 2016

Tuesday 19th July

Lab work

Biobrick characterization

K1372001 from clone 2 digestion

By Alice and Terrence

We made a digestion of K1372001 plasmid following this protocol. We used EcoR1 and Pst1 fast digest enzymes. After incubation, 3.3 mL of loading dye is added to digestion products. We put in wells 10µL of DNA ladder and 20 µL of digestion products. We set the eletrophoresis machine at 100V for 25min.

Digestion products expected were :

Band size (Bp)
Plasmid 1590
Insert 2029

Visualization

By

Get DNA Closer

By