Thursday 21^th July

Lab work

Biobrick characterization

K1372001 from clone 2 digestion

By Alice and Terrence

We made a digestion of K1372001 plasmid following this protocol. We used EcoR1 and Pst1 fast digest enzymes. After incubation, 3.3 mL of loading dye is added to digestion products. We put in wells 10µL of DNA ladder and 20 µL of digestion products. We set the eletrophoresis machine at 100V for 25min.

Digestion products expected were :

Migration of digested product of K1372001

Making Petri dishes of medium LB (with strepto or IPTG and Xgal

By Laetitia

A Petri dish with LB and streptomycin was made with:

• 20 mL of LB agar
• 10µL of streptomycin (initial concentration 100mg/mL)

We added IPTG and Xgal on 4 petri dishes of LB and Ampicilin. For each petri dish:

• 500µL of water
• 1µL of IPTG
• 1µL of Xgal

The solution was spreaded on petri dish

Liquid culture of pcl TAA, TAG and Tq

By Laetitia

2 clones of each petri dish were soaked in a tube of LB and Streptomycin.

6 tubes were made. For each tube:

• 1 mL LB
• 0.5 µL streptomycin

The tubes were incubated at 37°C and 180 rpm overnight.

Visualization

By

Get DNA Closer

By