Revision as of 12:48, 28 July 2016

Thursday 28^th July

By Mathilde and Laetitia

PCR was performed on 6 clones for each plasmid.

Thus, the PCR mix was done for 24 tubes following the usual protocol.

Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG.

PCR was done with a Tm at 57°C.

Each PCR Product was put to migrate on an agarose gel.

@@ Line 3: / Line 3: @@
 ==Lab work==
 ===Visualization===
-====Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009  ====
+====Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009====
-''By Laetitia ''
+''By Mathilde and Laetitia ''
-PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009.
+PCR was performed on 6 clones for each plasmid.
-Thus, the PCR mix was done for 12 tubes following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]].
+Thus, the PCR mix was done for 24 tubes following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]].
 Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG.