Thursday 28^th July

Lab work

Visualization

Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009

By Mathilde and Laetitia

PCR was performed on 6 clones for each plasmid.

Thus, the PCR mix was done for 24 tubes following the usual protocol.

Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG.

PCR was done with a Tm at 57°C.

Each PCR Product was put to migrate on an agarose gel.

Biobrick Characterization

BL21 electrocompetent cells in glycerol stock

By Charlène

1 mL of each clone cultured yesterday were put in 500µL of glycerol 60%. They were conserved at -30°C.