Difference between revisions of "Team:Paris Saclay/Notebook/July/29"
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The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | ||
| − | ==== ==== | + | ====High fidelity PCR on bacteria transformed with [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001]], [[Team:Paris_Saclay/Notebook/June/28#pPS16_002|pPS16_002]] and [[Team:Paris_Saclay/Notebook/June/28#pPS16_005|pPS16_005]] ==== |
| − | ''By '' | + | ''By Alice and Mathilde '' |
| + | |||
| + | A PCR with Q5® High-Fidelity 2X Master Mix was performed on clones selected after screening PCR made on July 28, following [[Team:Paris_Saclay/Experiments#Q5PCR|this protocol]].[[Team:Paris_Saclay/Experiments#primers|1151_pheoR and 1152_pheoF]] primers were used. Annealing temperature was 66°C and initial denaturation was 3 min. After amplification, 1 µL of loading dye was added to 5µL of each PCR products. Then 5 µL of this mix were put into a well of the gel. A last well contained 10µL of DNA ladder. PCR products were migrated 30min at 100V. | ||
| + | |||
| + | PCR products expected were : | ||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | !Plasmids | ||
| + | !Band size (bp) | ||
| + | |- | ||
| + | |pPS16_001 | ||
| + | |1017 | ||
| + | |- | ||
| + | |pPS16_002 | ||
| + | |1017 | ||
| + | |- | ||
| + | |pPS16_005 | ||
| + | |1017 | ||
| + | |} | ||
| + | |||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 14:51, 29 July 2016
