Difference between revisions of "Team:Paris Saclay/Notebook/August/1"
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gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
| + | ====gBlock 3.1 insertion in puc19==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | ||
| + | |||
| + | ==== DH5alp|pPS16_003, DH5alp|pPS16_003, DH5alp|pPS16_003 and DH5alp|pPS16_003 streak to recover colonies ==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | Checked DH5alp transformed with pPS16_003, pPS16_004, pPS16_006 and pPS16_007 were streaked on Pteri dishes containing LB + Amp (50µg/mL) + IPTG (0.1 µL/mL) + Xgal (0.25µL/mL) from glycerol cultures. | ||
Revision as of 13:29, 1 August 2016
