Difference between revisions of "Team:Paris Saclay/Notebook/August/2"

(Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 1<sup>st</sup> August= ==Lab work== ===Visualization=== ==== DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_00...")
 
(DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015)
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''By Charlène, Mathilde, Laetitia, Caroline and Léa''
 
''By Charlène, Mathilde, Laetitia, Caroline and Léa''
  
A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers.
+
A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] and using the universal puc19 primers.
  
 
==== Sequencing of the gBlock 4.2 ====
 
==== Sequencing of the gBlock 4.2 ====

Revision as of 09:32, 2 August 2016

Monday 1st August

Lab work

Visualization

DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015

By Charlène, Mathilde, Laetitia, Caroline and Léa

A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers.

Sequencing of the gBlock 4.2

By Caroline

The PCR products obtain with Q5 on pPS16_008 were send to sequencing.