Difference between revisions of "Team:Paris Saclay/Notebook/August/2"
(→DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
(→DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
||
| Line 6: | Line 6: | ||
''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ||
| − | A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] and using the universal puc19 primers. | + | A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] and using the universal puc19 [[Team:Paris_Saclay/Experiments#primers|primers]]. 6 clones for each transformation was tested. |
==== Sequencing of the gBlock 4.2 ==== | ==== Sequencing of the gBlock 4.2 ==== | ||
Revision as of 09:33, 2 August 2016
