Difference between revisions of "Team:Paris Saclay/Notebook/August/4"
(→Visualization) |
(→DNA Extraction of plasmids containing g blocks 1.1, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP) |
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| Line 3: | Line 3: | ||
==Lab work== | ==Lab work== | ||
===Visualization=== | ===Visualization=== | ||
| − | ==== DNA Extraction of | + | ==== DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN- ==== |
''By Caroline, Naiane, Mathilde and Laetitia'' | ''By Caroline, Naiane, Mathilde and Laetitia'' | ||
| Line 15: | Line 15: | ||
! Clone | ! Clone | ||
|- | |- | ||
| − | | | + | | pPS16_001 |
| 2, 9 | | 2, 9 | ||
|- | |- | ||
| − | | | + | | pPS16_002 |
| 4, 7, 11 | | 4, 7, 11 | ||
|- | |- | ||
| − | | | + | | pPS16_009 |
| 7 | | 7 | ||
|- | |- | ||
| − | | | + | | pPS16_003 |
| 4 | | 4 | ||
|- | |- | ||
| − | | | + | | DS-NMcasN- |
| 7, 11 | | 7, 11 | ||
|- | |- | ||
| − | | | + | | DS-TDcasN- |
| 1 | | 1 | ||
|- | |- | ||
| − | | | + | | DS-SPcasN- |
| 1 | | 1 | ||
|} | |} | ||
| Line 39: | Line 39: | ||
The extraction was performed using the kit. We followed this [[Team:Paris_Saclay/Experiments#DNA_extraction_using_the_Invitrogen_ChargeSwitch.C2.AE-Pro_Plasmid_Miniprep_Kit|protocol]]. | The extraction was performed using the kit. We followed this [[Team:Paris_Saclay/Experiments#DNA_extraction_using_the_Invitrogen_ChargeSwitch.C2.AE-Pro_Plasmid_Miniprep_Kit|protocol]]. | ||
| − | |||
==== Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP ==== | ==== Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP ==== | ||
Revision as of 13:46, 4 August 2016
