Difference between revisions of "Team:Paris Saclay/Notebook/August/4"
(→Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN-) |
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*700 µL of culture | *700 µL of culture | ||
*300 µL of glycerol (60%) | *300 µL of glycerol (60%) | ||
| + | |||
| + | ==== PCR Clean-up with the NucleoSpin kit ==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual [[Team:Paris_Saclay/Experiments#Purification|protocol]]. But, I forgot to diluted the Buffet NT3 with 100mL ethanol befor I began the purification and I did not obtain any result on the 0.8% agarose gel. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 14:59, 4 August 2016
