Difference between revisions of "Team:Arizona State/Description"

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<h2>REFERENCES</h2>
 
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
<li>References and sources to document your research.</li>
 
<li>Use illustrations and other visual resources to explain your project.</li>
 
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<h5>Advice on writing your Project Description</h5>
 
 
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h5>References</h5>
 
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
 
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<h5>Inspiration</h5>
 
<p>See how other teams have described and presented their projects: </p>
 
  
 
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Revision as of 20:14, 8 August 2016

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DESCRIPTION

The objective will be accomplished with a previously designed, flexible testing platform, in which the QS system is separated into two components designated the “Sender” and the “Receiver”. The HSL synthase is expressed in the Sender cell, while the inducible promoter and regulator are carried by a Receiver cell. When the Sender produces a signal, the HSL, it diffuses across cell membranes and activates the Receiver. In our current system, Receivers will express green fluorescent protein (GFP) in response to induction by Senders from different bacterial species. Currently, our team has built 10 senders and 7 receivers, several of which have been shown to be functional in E. coli. Testing for the remaining systems is still underway, and more receivers are still being cloned.

Our iGEM team is investigating the diverse applications that fit with our quorum sensing project. Some of the sub-projects include: investigating the Aub strain, which originates from unidentified soil bacterium, to decipher its organism of origin; making a “super quorum sensing” E. coli that is engineered to respond to wider variety of HSLs; more comprehensive characterization of HSLs produced by our Senders using mass spec; and building a genetic-based circuit model using Arduino or Logism. The latter is one of our several outreach projects, designed to increase future participation and interest in the ASU iGEM team.

MOTIVATION

Why we decided to work on this project: Homoserine lactone (HSL) quorum sensing (QS) is a type of communication that allows bacteria to monitor their population density for the purpose of controlling various group activities such as virulence or luminescence. Synthetic biologists have taken advantage of the simplicity of the QS system to incorporate signal-processing pathways into genetic circuits. This project aims to determine pathways with minimal overlap (“crosstalk”) and engineer QS modules as a flexible tool for building layered genetic circuits. Crosstalk occurs when when a single regulator is activated by multiple varieties of HSL molecules, impeding successful operation of complex genetic circuitry that uses multiple quorum sensing pathways. Homologous HSL networks have been identified in over 100 species of bacteria, but only four have been used in synthetic systems reported to date. Our goal is to expand the QS toolbox and enable the implementation of higher-order, complex genetic circuitry in synthetic biology.

GOLD MEDAL WORK

To be added

REFERENCES