Difference between revisions of "Team:Paris Saclay/Notebook/August/8"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
| − | = | + | =Monday 8<sup>st</sup> August= |
==Lab work== | ==Lab work== | ||
===Visualization=== | ===Visualization=== | ||
| − | + | ==== Q5 PCR on pPS16_006 and pPS16_007 ==== | |
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| − | ==== Q5 PCR on | + | |
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''By Caroline'' | ''By Caroline'' | ||
| − | The | + | The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#phusion|protocol]] adapted to 50µL and with a TM at 72°C. The specific [[Team:Paris_Saclay/Experiments#primers|primers]] for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET. No amplification were observed that was probably due to the fact that the extractions made on were eluted with water which is not working with this extraction kit. |
| + | ==== Liquid cultures of bacteria containing plasmid to extract ==== | ||
| + | ''By Caroline, Charlène, Terrence'' | ||
| − | + | In order to redo the extractions that did not working due to the use of water instead of the elution buffer. To do it, 5mL of LB were mixed with Ampicillin at 50µg/mL at put at 37°C and at 180rpm overnight. | |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 13:43, 9 August 2016
