Difference between revisions of "Team:Paris Saclay/Notebook/August/9"
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==Lab work== | ==Lab work== | ||
===Visualization=== | ===Visualization=== | ||
| + | ====Heat shock transformation==== | ||
| + | ''By Charlène and Terrence'' | ||
| − | + | For 1.2, 4.2, SgNm, SgSt1, Detection, FRB and FKBP gBlocks, the results of the sequencing were not as expected, so we transformed | |
| − | + | them a second time (in puc19). We followed the usual [[Team:Paris_Saclay/Experiments#Heat_shock_competent_cells|protocol]]. | |
| + | 50µL of cells were streaked on LB + Amp (50µg/mL) + IPTG + xGal. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 13:50, 9 August 2016
