Difference between revisions of "Team:Paris Saclay/Notebook/August/9"
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''By Charlène and Terrence'' | ''By Charlène and Terrence'' | ||
| − | For 1.2, 4.2, SgNm, SgSt1, Detection, FRB and FKBP gBlocks, the results of the sequencing were not as expected, | + | For 1.2, 4.2, SgNm, SgSt1, Detection, FRB and FKBP gBlocks, the results of the sequencing were not as expected.So, we transformed |
| − | + | pPS16_002, pPS16_008, pPS16_010, pPS16_011, pPS16_012, pPS16_013 and pPS16_014 in DH5a. We followed the usual [[Team:Paris_Saclay/Experiments#Heat_shock_competent_cells|protocol]]. | |
50µL of cells were streaked on LB + Amp (50µg/mL) + IPTG + xGal. | 50µL of cells were streaked on LB + Amp (50µg/mL) + IPTG + xGal. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 13:57, 9 August 2016
