Difference between revisions of "Team:Paris Saclay/Notebook/August/11"

(pPS16_010 extraction)
(pPS16_010 extraction)
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====[[Team:Paris_Saclay/Experiments#pPS16_010|pPS16_010]] extraction====
 
====[[Team:Paris_Saclay/Experiments#pPS16_010|pPS16_010]] extraction====
 
''By Alice''
 
''By Alice''
 +
 
After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z
 
After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z
 
[[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]]
 
[[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]]
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 15:06, 11 August 2016

Wednesday 10th August

Lab work

Visualization

Transformation of DH5a cells with pPS16_009

By Léa

Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual protocol.


pPS16_010 extraction

By Alice

After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following this protocol. After extraction, plasmids were migrated on a gel.z

Migration of FRB plasmid