Difference between revisions of "Team:Paris Saclay/Notebook/August/12"

(Extraction of puc19)
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Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]].
 
Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]].
They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water.  
+
They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.
  
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 12:53, 12 August 2016

Friday 12th August

Lab work

Visualization

PCR of pPS16_009

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

Extraction of puc19

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit. They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.