Difference between revisions of "Team:Paris Saclay/Notebook/August/12"

(Extraction of puc19)
(Lab work)
Line 16: Line 16:
  
 
They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.   
 
They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.   
 +
 +
 +
 +
====PCR====
 +
''By Naiane, Mahnaz and Terrence''
 +
 +
The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]]
 +
 +
 +
  
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 15:25, 12 August 2016

Friday 12th August

Lab work

Visualization

PCR of pPS16_009

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

Extraction of puc19

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.

They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.


PCR

By Naiane, Mahnaz and Terrence

The PCR was carried out following the usual protocol