Revision as of 15:35, 12 August 2016

Friday 12^th August

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.

They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.

By Naiane, Mahnaz and Terrence

A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRnaST, SgRnaNM and 1.2 using the usual protocol with these volumes :

We made 6 clones of each colonie except for Puc19 where we made 1 negative control.

@@ Line 19: / Line 19: @@
-====PCR====
+====PCR ====
 ''By Naiane, Mahnaz and Terrence''
-The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]]
+A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRnaST, SgRnaNM and 1.2 using the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]] with these volumes :
+{| class="wikitable"
+|-
+!Components
+!Volume
+|-
+|10X DreamTaq Green Buffer
+|2.5µL
+|-
+|dNTP (10mM)
+|1µL
+|-
+|Primers mix (10µM each)
+|1µL
+|-
+|DreamTaq DNA polymerase
+|0.15µL
+|-
+|Nuclease-free water
+|up to 25µL
+|-
+!Total volume
+!25µL
+|}
+We made 6 clones of each colonie except for Puc19 where we made 1 negative control.