Revision as of 15:38, 12 August 2016

Friday 12^th August

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.

They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.

By Naiane, Mahnaz and Terrence

A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2 using the usual protocol with these volumes :

We made 6 clones of each colonie except for Puc19 where we made 1 negative control.

@@ Line 19: / Line 19: @@
-====Dreamtaq PCR on puc19, detection, FRB, FKBP, SgRnaST, SgRnaNM and 1.2====
+====Dreamtaq PCR on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2====
 ''By Naiane, Mahnaz and Terrence''
-A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRnaST, SgRnaNM and 1.2 using the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]] with these volumes :
+A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2 using the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]] with these volumes :
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