Difference between revisions of "Team:Hong Kong HKUST/Danson"

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   <h1 align=“left”; style="color:#542521;font-family: marker felt;" >Abstract</h1>
 
   <h1 align=“left”; style="color:#542521;font-family: marker felt;" >Abstract</h1>
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   <p style="font-family:osaka;">Tri-stable switch is a biological device that could perform three discrete, but alternating, steady states driven by three different repressible promoters. The presence of a transient pulse of inducer allows effective state shifting, while signal interference is prevented. In order to enhance the specificities towards inducers, improvements were made based on the Brown’s tri-stable switch model in 2006. This year, three well-characterized repressible promoters are used: phlFp, tetp, and lacp. The whole construct is divided into three parts, and each contains one functional system with two protein coding sequences(CDS), creating an interconnected tri-stable toggle switch. Moreover, mathematical modelling is applied to predict and verify the consistency of the experimental results. At present, it is by far possible and practical to apply the switch in biosensing which could be achieved by developing a combinatorial circuit of promoters and CDS. With its advantageous characteristics, it is foreseeable that the switch could be applied in a wider spectrum of fields in the near future, for example, biocomputational system and diseases diagnostic.</p>
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   <p style="font-family:osaka;">The Tristable switch was derived from advanced studies of other synthetic networks like toggle switches and oscillators. We think it would be helpful to create a synthetic network which produces distinct signals under specific induction. We hope to create a biological device that could perform three discrete, but alternating, steady states driven by three different repressible promoters. The presence of a transient pulse of inducer allows effective state shifting, while preventing signal interference.
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During data-mining, we noticed that iGEM Team Brown University has tried to build tristable switches in 2006 and 2007 but had dissatisfactory outputs. Therefore, we troubleshot and investigated improvements that can be applied to their design.</p>
 
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Revision as of 04:02, 16 August 2016

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Abstract

The Tristable switch was derived from advanced studies of other synthetic networks like toggle switches and oscillators. We think it would be helpful to create a synthetic network which produces distinct signals under specific induction. We hope to create a biological device that could perform three discrete, but alternating, steady states driven by three different repressible promoters. The presence of a transient pulse of inducer allows effective state shifting, while preventing signal interference. During data-mining, we noticed that iGEM Team Brown University has tried to build tristable switches in 2006 and 2007 but had dissatisfactory outputs. Therefore, we troubleshot and investigated improvements that can be applied to their design.