Revision as of 14:29, 16 August 2016

Friday 12^th August

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

Electrophoresis of PCR products, using primers IPS 83 and IPS 84 on pPS16_016.

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.

They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.

By Naiane, Mahnaz and Terrence

A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2 using the usual protocol with these volumes :

We made 6 clones of each colonie except for Puc19 where we made 1 negative control.

@@ Line 9: / Line 9: @@
 A Dreamtaq PCR was performed on pPS16_009 using the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]].
 Primers: IPS83 ans IPS84
+[[File:T--Paris_Saclay--120826_visualization_PCRGFP.jpeg|400px|thumb|right|Electrophoresis of PCR products, using primers IPS 83 and IPS 84 on pPS16_016.]]
 ====Extraction of puc19====