Difference between revisions of "Team:Pretoria UP/Description"

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        <title>WATTS-APTAMER - UP_PRETORIA iGEM</title>
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<a href="https://2016.igem.org/Team:Pretoria_UP" class="navbar-brand">UP_PRETORIA 2016</a>
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                    <ul class="nav navbar-nav navbar-right navbar-uppercase">
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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                          <li>
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                            <a href="https://2016.igem.org/Team:Pretoria_UP">
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                            Home
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                            </a>
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                        <li>
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Description">
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                            Project Description
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                            </a>
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                            <a href="https://2016.igem.org/Team:Pretoria_UP#parts">
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                            Parts
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Measurement">
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                            Results
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                            </a>
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Modeling">
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                            Modeling
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                            </a>
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Interlab">
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                            Interlab Study
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Collaborations">
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                            Collaboration
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                            Human Practices
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                            <a href="https://2016.igem.org/Team:Pretoria_UP#ref">
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                            References
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Medal_Criteria">
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                            Medal Criteria
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                            <a href="https://2016.igem.org/Team:Pretoria_UP/Team">
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                            Team
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                                                <p class="h2WM">Project Description</p>
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                                            </div>
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<li> A clear and concise description of your project.</li>
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                                                <p>The world population consumes approximately 3500 kWh/y/capita, increasing the demand for clean alternative energy. Recent improvements of photo-bioelectrochemical cells (PBEC), which harness electrons from photosynthesis to generate electricity, include synthetic attachment of chloroplast thylakoids to graphene electrodes. However, current attachment techniques require costly chemically synthesized linkers and PBECs are not yet efficient enough for industrial energy generation. In this project, DNA aptamers were designed and evaluated as low-cost biological linkers to tether plant photosystem II (PSII) complexes to graphene foam electrodes. Systematic Evolution of Ligands by EXponential enrichment (SELEX), together with software developed by team Heidelberg 2015 (MAWS and JAWS) were used to develop PSII- and graphene-binding DNA aptamer candidates. This project aims to improve the attachment and orientation of the PSII complex to the graphene electrode for higher electron transfer efficiency, and serves as a prototype for the in planta expression of RNA aptamers for self-assembling thylakoid attachment.
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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<a href=""></a><p>Photo-bioelectrochemical cells hold great potential as clean, alternative energy sources. A major barrier is the attachment, efficiency and cost of the system, making scalability unfeasible. Particularly, synthetic linkers used for thylakoid attachment to electrodes are expensive and difficult to manufacture at sufficient scale.
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</p><p>Our aim with this project is to design and construct an optimized photo-bioelectrochemical cell using an in planta RNA aptamer synthetic biology strategy for self-assembling attachment of plant thylakoids to graphene-coated anodes.
 
</p>
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p>References:</p>
  
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<p>1: Elowitz, Michael B., et al. "Stochastic gene expression in a single cell." Science 297.5584 (2002): 1183-1186.</p>
  
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<p>2: Raser, Jonathan M., and Erin K. O'Shea. "Noise in gene expression: origins, consequences, and control." Science 309.5743 (2005): 2010-2013.
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<p>3: Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183. </p>
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</div>
  
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                                  2016 UP_Pretoria iGEM
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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action=raw&ctype=text/javascript"></script>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Revision as of 19:41, 16 August 2016

WATTS-APTAMER - UP_PRETORIA iGEM

Project Description

The world population consumes approximately 3500 kWh/y/capita, increasing the demand for clean alternative energy. Recent improvements of photo-bioelectrochemical cells (PBEC), which harness electrons from photosynthesis to generate electricity, include synthetic attachment of chloroplast thylakoids to graphene electrodes. However, current attachment techniques require costly chemically synthesized linkers and PBECs are not yet efficient enough for industrial energy generation. In this project, DNA aptamers were designed and evaluated as low-cost biological linkers to tether plant photosystem II (PSII) complexes to graphene foam electrodes. Systematic Evolution of Ligands by EXponential enrichment (SELEX), together with software developed by team Heidelberg 2015 (MAWS and JAWS) were used to develop PSII- and graphene-binding DNA aptamer candidates. This project aims to improve the attachment and orientation of the PSII complex to the graphene electrode for higher electron transfer efficiency, and serves as a prototype for the in planta expression of RNA aptamers for self-assembling thylakoid attachment.

Photo-bioelectrochemical cells hold great potential as clean, alternative energy sources. A major barrier is the attachment, efficiency and cost of the system, making scalability unfeasible. Particularly, synthetic linkers used for thylakoid attachment to electrodes are expensive and difficult to manufacture at sufficient scale.

Our aim with this project is to design and construct an optimized photo-bioelectrochemical cell using an in planta RNA aptamer synthetic biology strategy for self-assembling attachment of plant thylakoids to graphene-coated anodes.

References:

1: Elowitz, Michael B., et al. "Stochastic gene expression in a single cell." Science 297.5584 (2002): 1183-1186.

2: Raser, Jonathan M., and Erin K. O'Shea. "Noise in gene expression: origins, consequences, and control." Science 309.5743 (2005): 2010-2013.

3: Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183.