Difference between revisions of "Team:Paris Saclay/Notebook/August/17"
(→Culture of BL21) |
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3 clones from each transformation condition were grown in 500µL of LB, streptomycin (50µg/mL) and chloramphenicol (30µg/mL). | 3 clones from each transformation condition were grown in 500µL of LB, streptomycin (50µg/mL) and chloramphenicol (30µg/mL). | ||
Then each solution was split into 3x150µL and 350µL of mix of LB + streptomycin (50µg/mL) + chloramphenicol (30µg/mL) were added to each culture. | Then each solution was split into 3x150µL and 350µL of mix of LB + streptomycin (50µg/mL) + chloramphenicol (30µg/mL) were added to each culture. | ||
| − | Salicylate (main solution at 10mM) was added to obtain the following concentrations : 0, 0.03mM and | + | Salicylate (main solution at 10mM) was added to obtain the following concentrations : 0, 0.03mM and 1mM. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:21, 17 August 2016
