Difference between revisions of "Team:Paris Saclay/Notebook/August/19"
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| + | ====Plasmids extraction==== | ||
| + | "By Terrence, Alice and Manhaz" | ||
| + | ==== Colony and Extraction product DreamTaq PCR==== | ||
| + | "By Léa and Naiane" | ||
| + | |||
| + | A Colony PCR was performed on Dh5a transformed with PJet containing sgRNA Nm or 1.2 gBlocks. | ||
| + | sgRNA Nm clones 6, 7, 8, 10, 11 and 12 were screened, and 1.2 clones 13 to 18 were screened and plated on Petri dish containing solid LB and Ampicilin. | ||
| + | |||
| + | In the meantime, a DreamTaq PCR was performed on plasmids extractions listed above, using the same PCR mix. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 09:56, 19 August 2016
