(Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 22<sup>th</sup> August= ==Lab work== ===Visualization=== ====Low fidelity Dreamn Taq PCR of pPS16_016==== ''By Mathilde'' Clone...") |
(→Monday 22th August) |
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* 1,5µL of Green Buffer | * 1,5µL of Green Buffer | ||
* 1µL of dNTPs | * 1µL of dNTPs | ||
− | * 1µL of each primers | + | * 1µL of each primers IPS83 and IPS84 |
* 0,13µL of Dream Taq Polymerase | * 0,13µL of Dream Taq Polymerase | ||
* 19,37 µL of H20 | * 19,37 µL of H20 | ||
− | Colonies were put into sterile water, and were | + | Colonies were put into sterile water, put through 5 minutes of denaturation before the addition of the mix. |
+ | Annealing temperature was 57°c. | ||
+ | After amplification, 1µL of each PCR product and 10µL of the DNA purple ladder were palced in wells and migrated at 100V for 30min. | ||
+ | |||
+ | ====Purification on gel of gBlocks 3 and 4==== | ||
+ | ''Terrence and Mathilde'' | ||
+ | |||
+ | After amplification, 50µL of each PCR product diluted 6 tumes with 10 µL of the gel loadding dye, and 10µL of the DNA purple ladder were placed in wells and migrated at 100V for 30min. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 13:18, 22 August 2016