Difference between revisions of "Team:Paris Saclay/Notebook/August/25"
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==Lab work== | ==Lab work== | ||
===Visualization=== | ===Visualization=== | ||
| − | ====Extraction ==== | + | ====Extraction of plasmids containing sgRNA Nm, fragment 3 + 4 (from the HIfi assembly mix) , GFP1-9==== |
''By Terrence'' | ''By Terrence'' | ||
| + | The extraction was carried out following the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|usual protocol]]. | ||
| + | The plasmid PSB1C3 with the insert : | ||
| + | - SgRNA Nm were extracted from the clone 5 | ||
| + | - Fragment 3 + 4 were extracted from the clone 6 | ||
| + | - GFP1-9 were extracted from the clone 4 | ||
| + | |||
| − | ====Digestion==== | + | |
| + | |||
| + | ====Digestion of PSB1C3 containing the fragment 3 + 4 assembly and PSB1C3 with GFP1-9's insert.==== | ||
''By Terrence'' | ''By Terrence'' | ||
| + | Extracted plasmids were digest with XbaI and PstI, following [[Team:Paris_Saclay/Experiments#PlasmidDigestion|usual protocol]]. | ||
Revision as of 12:47, 25 August 2016
