Team:Arizona State/Experiments
Protocols
[1A] Polymerase Chain Reaction
PCR is used to rapidly amplify certain segments of DNA that are selected via designed primers. This protocol was used to accomplish a variety of goals, from extraction of parts from a cloning vector to the addition of restriction cut sites at the ends of parts.
- Defrost template, 10x Master Mix and primers
- Determine desired total working volume (~50uL)
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