Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 26th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Purification of pPS16-003 and pPS16_004 ligation products 1.1.1.2 Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product 1.1.1.3 Purification of fragments 1(16-001 + pPS16_002) and 2 (16-003 + pPS16_004) Friday 26th August Lab work Visualization Purification of pPS16-003 and pPS16_004 ligation products By Mathilde Ligation products pPS16-003 and pPS16_004 frome the day before were purificated according to the usual protocol Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product By Mathilde The Q5 PCR was conducted following the exact same protocol than the 25/08/2016 on the ligation product from the day before. Migration Results The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified. Purification of fragments 1(16-001 + pPS16_002) and 2 (16-003 + pPS16_004) By Mathilde The purification was made following the usual protocol. Each of those purification products were quantified on nanodrop. Segment DNA quantity (ng/µL) 260/230 260/280 1 84,20 1,25 1,84 2 169,88 1,29 1,83
By Mathilde
Ligation products pPS16-003 and pPS16_004 frome the day before were purificated according to the usual protocol
The Q5 PCR was conducted following the exact same protocol than the 25/08/2016 on the ligation product from the day before.
The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified.
The purification was made following the usual protocol. Each of those purification products were quantified on nanodrop.
