Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Monday 5th September 1.1 Lab work 1.1.1 Visualization 1.1.1.1 fragments 1.1 with 1.2 Ligation AND 2.1 with 2.2 Ligation Monday 5th September Lab work Visualization fragments 1.1 with 1.2 Ligation AND 2.1 with 2.2 Ligation By Mahnaz Fragment 1.1 and 1.2 were ligated together as following 4µL of Fragment 1.1 PCR product from the 02/09/2016 4µM of Fragment 1.2 PCR product from 02/09/2016 1µL of Buffer T4 10X 1µL of ligase T4 enzyme Fragment 2.1 and 2.2 were ligated together as following 4µL of Fragment 2.1 PCR product from the 02/09/2016 4µM of Fragment 2.2 PCR product from 02/09/2016 1µL of Buffer T4 10X 1µL of ligase T4 enzyme The ligation product was put at room temperature for 1 hour.
By Mahnaz
Fragment 1.1 and 1.2 were ligated together as following
Fragment 2.1 and 2.2 were ligated together as following
The ligation product was put at room temperature for 1 hour.
