Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 23rd September 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Glycerol stocks of clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) 1.1.1.2 Plasmids extraction of clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) 1.1.1.3 Digestion of plasmids extracted from clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) 1.1.1.4 Glycerol stocks of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) 1.1.1.5 Plasmids extraction of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) Friday 23rd September Lab work Visualization Glycerol stocks of clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) "By Maxence, Mahnaz & Coline" The glycerol stock of the bacteria with the following plasmids were made. pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X 500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C. Plasmids extraction of clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) "By Maxence, Mahnaz & Coline" The following plasmids were extracted using the "Charge Switch-Pro Plasmid Miniprep" kit: pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X pPS16_022 (GFP 1.9) clone X Digestion of plasmids extracted from clones X, X, X and X containing FRB - GFP 11 - GFP 1.9 in pSB1C3 (pPS16_022) "By Maxence, Mahnaz & Coline" To assess if DH5a cells were transformed with the good plasmid, plasmid digestion was run with XbaI as following: 1 µL of extracted plasmid 1 µL of buffer 1 µL of restriction enzyme XbaI 7 µL of water A control was done with 1 µL of non-digested plasmid. GEL Glycerol stocks of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) "By Maxence, Mahnaz & Coline" The glycerol stock of the bacteria with the following plasmids were made. pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X 500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C. Plasmids extraction of clones X, X, X and X containing corrected dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) "By Maxence, Mahnaz & Coline" The following plasmids were extracted using the "Charge Switch-Pro Plasmid Miniprep" kit: pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X pPS16_017 (dCas9 ST - GFP 11) clone X
"By Maxence, Mahnaz & Coline"
The glycerol stock of the bacteria with the following plasmids were made.
500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C.
The following plasmids were extracted using the "Charge Switch-Pro Plasmid Miniprep" kit:
To assess if DH5a cells were transformed with the good plasmid, plasmid digestion was run with XbaI as following:
A control was done with 1 µL of non-digested plasmid.
GEL
