Team:Paris Saclay/Strategy

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In order to answer our question, we choose to create a new technology to bring two different DNA regions closer. As a results, we have designed a linking tool and a visualization tool.

Here, we will expose you our experimental strategy, as well as, the biobricks we have designed to do so.

Linking tool construction

Visualization tool construction

Characterization strategy

Tripartite Split-GFP and FRB/FKBP12 dimerization systems
Assessment of the minimal distance to have fluorescence
Assessment of the DNA regions brought closer
Gene expression tests