Team:Paris Saclay/Strategy

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In order to answer our question, we choose to create a new technology to bring two different DNA regions closer. As a results, we have designed a linking tool and a visualization tool.

Here, we will expose you our experimental strategy, as well as, the biobricks we have designed to do so.

Linking tool construction

Visualization tool construction

T--Paris Saclay--visualization biobricks.jpeg
T--Paris Saclay--composite visualization biobrick.jpeg

Characterization strategy

Tripartit Split-GFP and FRB/FKBP12 dimerization systems
Assessment of the minimal distance to have fluorescence
Assessment of the DNA regions brought closer
Gene expression tests