Team:Peking/Notebook

Notebook.

In this page, you can view how we worked on our project.

Diary

Week 1 (6/26/2016-7/02/2016)

• We transformed BioBricks from the distribution:
• BBa_J33201 (arsR)
• BBa_K516030 (mRFP1 with RBS and terminator)
• Colonies were used to inoculate overnight cultures.
• Plasmids were isolated using a miniprep kit.

Week 2 (4/20/2015-4/26/2015)

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Week 3 (4/20/2015-4/26/2015)

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Week 4 (4/20/2015-4/26/2015)

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Week 5 (4/20/2015-4/26/2015)

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Week 6 (4/20/2015-4/26/2015)

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Week 7 (4/20/2015-4/26/2015)

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Week 8 (4/20/2015-4/26/2015)

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Week 9 (4/20/2015-4/26/2015)

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Protocols

SDS-Pages

Ligation

Temperature Gradient Experiment

PCR

Purification of Recombinant Proteins

Transformation

Testing Adsorption Capacity of Protein

Agarose Gel Electrophoresis

Biotin-associated Experiment

Gel Extraction

Western Blot

pH Gradient Experiment