Team:Paris Saclay/Notebook/October/6

Thursday 6th October

Lab work

Visualization

Plasmids extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4 and FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6

By Maxence & Victor

The following plasmids were extracted using the "Charge Switch-Pro Plasmid Miniprep" kit:

  • pPS16_018 (FKBP - GFP 10) clone 6
  • pPS16_019 (FRB - GFP 11) clone 4

After extraction, 5 µL of each plasmids and 20 µL of DNA ladder were placed in wells and migrated at 100V during 30 min in order to assess their concentration.