Team:Alverno CA/Notebook
Notebook
Week of June 6th (Limited amount of notes taken)
- Lots of Designing Parts and Primers
- Wiki Work
Week of June 13th:
- Cleaning Restriction Digest and Ligation Done
- Ampicillin plates made
- Ampicillin stock made
- Transform RFP plasmid
- Design Parts and Primers
- Wiki Work
Week of June 20th
- Transformation Done (probably GFP)
- RFP and GRP colonies
- PCR for RFP Coding Gene
- Design Parts and Primers
- Wiki Work
Week of June 27th
- PCR Done for promoters, terminators, some UC parts
- Primer Dilutions Done
- Gel Run with PCR product from Week of June 20th (RFP)
- Miniprep kit for RFP Bba
- TBE Buffer 10x and 1x made
- PCR Purification done on Promoters and Terminators
- Wiki Work
Week of July 4th
- PCR of Vectors, some UC parts
- Primer Dilutions
- Gel Run UC16-18 and UC2 (UC18 ran twice)
- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)
- Plate Reader
- Wiki Work
Week of July 11th
- Learn how to use Benchling
- Primers Diluted
- Parts Diluted
- PCR done on P1ab, P2ab, T3ab, T4ab
- Gel Run on P1ab, P2ab, T3ab, T4ab
- PCR Clean up done on P1ab, P2ab, T3ab, T4ab
- Chloramphenicol plates made
- Wiki Work
Week of July 18th
- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)
- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d
- Transformation Done
- Chloramphenicol plates made
- Parts dilutions Done
- PCR of GG Assemblies
- PCR of UC3a-UC9a
- Wiki Work
Week of July 25th
- Set up Plate reader
- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts
- PCR Purification of UC3-9, V1a & V2a
- Picked Colonies
- Transformation Protocol for GG1-8
- Gel made & Gel made w/ SYBR safe
- PCR of GG Assemblies
- PCR for V1a and V2a, UC7-9
- Dilutions Done
- Gel Run for UC7-9
- Golden Gate Assembly GG9-16
- Wiki Work
Week of August 1st
- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23
- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12
- PCR Purification for UC7-8
- Parts Dilutions done UC2 & UC3-6 & UC7-8
- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23
- Fixed Thermocycler
- Gels Made
- Agar Plates made w/ Kan
- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26
- Transformation with GG12
- Ludox Calibration
- Test Fluorescence of Plasmids
- Wiki Work
Week of August 8th
- Cell Measurement
- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR
- PCR Check for GG24-26 & GG27-28
- Made Gel
- Transformation with GG24-26 & GG27-28
- Golden Gate Assembly GG27-28
- Colony PCR Check of GG24-26
- Primer Dilutions
- Plasmid DNA Purification
- LB Kan Broth
- Wiki Work
Week of August 15th
- Lab Meet-up with UCL/LC/CV
- Colony PCR Check for GG libraries 27-28
- Gels Made
- Gel Run for GG Library 28
- Grow Cultures GG27
- Learn Python
- Check Fluorescence for GG28 & GG27
- PCR UC14a
- Purification of Plasmid DNA
- Wiki Work
Week of August 22nd
- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36
- Gels Made
- PCR UC13a and UC15a (Done twice) & UC1
- PCR Purification of UC14 & UC13/15 & UC1
- Golden Gate Assembly for GG29-36
- PCR Check for GG29-36
- LB Kan Broth Made
- Dilution of UC1
- Control run in Plate Reader
- Wiki Work
Week of August 29th
- Make Gels
- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a
- Golden Gate Assembly GG37-52 & GG53-60
- PCR Check GG37-52 & GG53-60
- PCR Check Gel run for GG37-52
- PCR CDS1a & CDS2a
- PCR Purification of CDS1a & CDS2a
- Talk with iGEM Peshawar
- LB Kan Plates Made
- Wiki Work
Week of September 5th
- iGEM Team Meeting
- PCR Purification of CDS1a & CDS2a and UC1
-
- Transformation was done with GG53-60
- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64
- Gels made
- Dilution of CDS1a & CDS2a
- Golden Gate Assembly GG61-64
- PCR of UC1
- LB Kan Plates Made
- PCR Check for GG61-64
- TBE 10x Buffer
- Wiki Work
Week of September 12th
- Talked with UCL iGEM Team
- LV-LC-CV meet-up
- Google Hangouts with Lambert Georgia iGEM team
- Dilution of UC1a
- Transformation of GG29-36
- Golden Gate Assembly GG65-72
- PCR GG61-64
- PCR Check GG61-64
- TX-TL of GFP (+ control)
- Outreach workshop with Middle school
- Wiki Work
Week of September 19th
- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE
- Run Gel for GG61-64 (done twice) and GG65-72
- LB Kan Plates Made
- Gels Made
- TX-TL GFP Re-run
- Gel Run for UC1 and GG61-64
- Control Test ran using only the ladder for Gels
- TX-TL W & M plasmids
- PCR U3a
- Dilution of Primers
- Wiki Work
Week of September 26th
- PCR Purification of V3a
- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94
- PCR Check GG73-74 and GG75-82
- Dilution V3a
- Run Gel V3a
- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74
- TX-TL re-run for W & M plasmids
- Colony PCR GG61-64 and GG65-72
- LB Chloramphenicol plates & LB Kanamycin plates
- Wiki Work
Week of October 3rd
- Middle School Visit
- LB Broth made
- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab
- Dilution V3a
- PCR Check GG83-90
- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab
- TX-TL re-run for W & M plasmids w/ Replicates
- Gels Made
